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purelink mirna isolation kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher purelink mirna isolation kit
    Purelink Mirna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/purelink+mirna+isolation+kit/purelink+mirna+isolation+kit/pm40409685-199-9-16
    Average 90 stars, based on 1 article reviews
    purelink mirna isolation kit - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Prospective Breast Cancer Biobanking Project
    Article Snippet: MiRNA and mRNA will be profiled using PureLinkTM miRNA Isolation Kit (Invitrogen), Dynabeads mRNA direct micro kit (Ambion),) and Ion total RNA-seq kit V2 (Life Technologies) on our Ion Proton NGS instrument.

    Article Title: The molecular motor Myosin 5B and its folding chaperone UNC45A are decreased in colorectal cancer.
    Article Snippet: To isolate miRNA from cell lines and organoids, the PureLink miRNA 439 isolation kit was utilized (ThermoFisher #K157001).

    Article Title: Inhibition of the chemokine receptors CXCR1 and CXCR2 synergizes with docetaxel for effective tumor control and remodeling of the immune microenvironment of HPV-negative head and neck cancer models
    Article Snippet: Cells were cultured in complete culture media supplemented with recombinant IL-8 (1 ng/mL, R&D Systems, Minneapolis, MN) and either DMSO or SX-682 (5 μM) for 24 h (UM-SCC-11B and UM-SCC-22B) or 48 h (UM-SCC-74B).

    Isolation:

    Article Title: Decoding Codon Bias: The Role of tRNA Modifications in Tissue-Specific Translation
    Article Snippet: .. Tissues were homogenized with QIAzol (QIAGEN, Hilden, Germany, #79306) and small RNA was isolated using a Purelink miRNA Isolation Kit (Thermo Fisher, Waltham, MA, USA, #K157001). .. For small RNA sequencing and ribosome profiling, three mice were perfused using ice-cold phosphate-buffered saline with 100 μg/mL cycloheximide (Sigma, St. Lousi, MO, USA, #01810).

    Article Title: Synergistic effects of tRNA modification defects in Escherichia coli K12
    Article Snippet: .. Pellets were resuspended in 1 mL of Trizol (Life Technologies) and agitated on a rocking shaker for 2-4 h. Small RNA was extracted using a Purelink miRNA Isolation kit (Invitrogen) and RNA eluted in 50 μL of RNase-free water. .. Following RNA quantification by Nanodrop, samples with A 260 /A 230 ratios <1.6 were cleaned using an RNA Clean and Concentrator Kit (Norgen Biotek).

    Article Title: The ubiquitous pyridoxal 5′‐phosphate‐binding protein is also an RNA ‐binding protein
    Article Snippet: Total RNA was isolated from BW25113 E. coli or human MDA‐MB‐231 and HAP1 cell cultures using the NucleoSpin RNA kit from Macherey and Nagel (Hoerdt, France). .. Low molecular weight RNA was purified from BW25113 E. coli cells using PureLink miRNA Isolation kit (Invitrogen, Waltham, MA, USA). .. RNA bound to proteins was purified using a total RNA purification kit from Norgen Biotek (Thorold, Ontario, Canada).

    Article Title: Mechanism of Catalysis and Substrate Binding of Epoxyqueuosine Reductase in the Biosynthetic Pathway to Queuosine-Modified tRNA.
    Article Snippet: Post-transcriptional modifications at the anticodon stem-loop of tRNAs are key to the translation function.. Metabolic pathways to these modifications often incorporate complex enzymology.. A notable example is the hypermodified nucleoside, queuosine, found at the wobble position of Asn, Asp, His, and Tyr encoding tRNAs.

    Article Title: Inhibition of the chemokine receptors CXCR1 and CXCR2 synergizes with docetaxel for effective tumor control and remodeling of the immune microenvironment of HPV-negative head and neck cancer models.
    Article Snippet: Amplification and inhibition of microRNA Cells were cultured in complete culture media supplemented with recombinant IL-8 (1 ng/mL, R&D Systems, Minneapolis, MN) and either DMSO or SX-682 (5 μM) for 24 h (UM-SCC-11B and UM-SCC-22B) or 48 h (UMSCC-74B). .. Micro-RNA was purified using the PureLink miRNA Isolation Kit (Invitrogen), according to the manufacturer’s instructions; next, reverse transcription was performed with a TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems) in conjunction with miR-specific primers for hsa-mir-200a (ID: 000502), hsamir-200b (ID: 002251), hsa-mir-200c (ID: 002300), and snoRNA-135 (ID: 001230) (TaqMan MicroRNA Assay, Applied Biosystems). cDNA was amplified in triplicate using TaqMan Master Mix with no AMP UNG (ThermoFisher Scientific) in an Applied Biosystems 7500 RealTime PCR System (ThermoFisher Scientific). miRNA expression was normalized to snoRNA-135 by calculating 2−ΔCt. ..

    Molecular Weight:

    Article Title: The ubiquitous pyridoxal 5′‐phosphate‐binding protein is also an RNA ‐binding protein
    Article Snippet: Total RNA was isolated from BW25113 E. coli or human MDA‐MB‐231 and HAP1 cell cultures using the NucleoSpin RNA kit from Macherey and Nagel (Hoerdt, France). .. Low molecular weight RNA was purified from BW25113 E. coli cells using PureLink miRNA Isolation kit (Invitrogen, Waltham, MA, USA). .. RNA bound to proteins was purified using a total RNA purification kit from Norgen Biotek (Thorold, Ontario, Canada).

    Purification:

    Article Title: The ubiquitous pyridoxal 5′‐phosphate‐binding protein is also an RNA ‐binding protein
    Article Snippet: Total RNA was isolated from BW25113 E. coli or human MDA‐MB‐231 and HAP1 cell cultures using the NucleoSpin RNA kit from Macherey and Nagel (Hoerdt, France). .. Low molecular weight RNA was purified from BW25113 E. coli cells using PureLink miRNA Isolation kit (Invitrogen, Waltham, MA, USA). .. RNA bound to proteins was purified using a total RNA purification kit from Norgen Biotek (Thorold, Ontario, Canada).

    Article Title: Inhibition of the chemokine receptors CXCR1 and CXCR2 synergizes with docetaxel for effective tumor control and remodeling of the immune microenvironment of HPV-negative head and neck cancer models.
    Article Snippet: Amplification and inhibition of microRNA Cells were cultured in complete culture media supplemented with recombinant IL-8 (1 ng/mL, R&D Systems, Minneapolis, MN) and either DMSO or SX-682 (5 μM) for 24 h (UM-SCC-11B and UM-SCC-22B) or 48 h (UMSCC-74B). .. Micro-RNA was purified using the PureLink miRNA Isolation Kit (Invitrogen), according to the manufacturer’s instructions; next, reverse transcription was performed with a TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems) in conjunction with miR-specific primers for hsa-mir-200a (ID: 000502), hsamir-200b (ID: 002251), hsa-mir-200c (ID: 002300), and snoRNA-135 (ID: 001230) (TaqMan MicroRNA Assay, Applied Biosystems). cDNA was amplified in triplicate using TaqMan Master Mix with no AMP UNG (ThermoFisher Scientific) in an Applied Biosystems 7500 RealTime PCR System (ThermoFisher Scientific). miRNA expression was normalized to snoRNA-135 by calculating 2−ΔCt. ..

    Reverse Transcription:

    Article Title: Inhibition of the chemokine receptors CXCR1 and CXCR2 synergizes with docetaxel for effective tumor control and remodeling of the immune microenvironment of HPV-negative head and neck cancer models.
    Article Snippet: Amplification and inhibition of microRNA Cells were cultured in complete culture media supplemented with recombinant IL-8 (1 ng/mL, R&D Systems, Minneapolis, MN) and either DMSO or SX-682 (5 μM) for 24 h (UM-SCC-11B and UM-SCC-22B) or 48 h (UMSCC-74B). .. Micro-RNA was purified using the PureLink miRNA Isolation Kit (Invitrogen), according to the manufacturer’s instructions; next, reverse transcription was performed with a TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems) in conjunction with miR-specific primers for hsa-mir-200a (ID: 000502), hsamir-200b (ID: 002251), hsa-mir-200c (ID: 002300), and snoRNA-135 (ID: 001230) (TaqMan MicroRNA Assay, Applied Biosystems). cDNA was amplified in triplicate using TaqMan Master Mix with no AMP UNG (ThermoFisher Scientific) in an Applied Biosystems 7500 RealTime PCR System (ThermoFisher Scientific). miRNA expression was normalized to snoRNA-135 by calculating 2−ΔCt. ..

    TaqMan microRNA Assay:

    Article Title: Inhibition of the chemokine receptors CXCR1 and CXCR2 synergizes with docetaxel for effective tumor control and remodeling of the immune microenvironment of HPV-negative head and neck cancer models.
    Article Snippet: Amplification and inhibition of microRNA Cells were cultured in complete culture media supplemented with recombinant IL-8 (1 ng/mL, R&D Systems, Minneapolis, MN) and either DMSO or SX-682 (5 μM) for 24 h (UM-SCC-11B and UM-SCC-22B) or 48 h (UMSCC-74B). .. Micro-RNA was purified using the PureLink miRNA Isolation Kit (Invitrogen), according to the manufacturer’s instructions; next, reverse transcription was performed with a TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems) in conjunction with miR-specific primers for hsa-mir-200a (ID: 000502), hsamir-200b (ID: 002251), hsa-mir-200c (ID: 002300), and snoRNA-135 (ID: 001230) (TaqMan MicroRNA Assay, Applied Biosystems). cDNA was amplified in triplicate using TaqMan Master Mix with no AMP UNG (ThermoFisher Scientific) in an Applied Biosystems 7500 RealTime PCR System (ThermoFisher Scientific). miRNA expression was normalized to snoRNA-135 by calculating 2−ΔCt. ..

    Amplification:

    Article Title: Inhibition of the chemokine receptors CXCR1 and CXCR2 synergizes with docetaxel for effective tumor control and remodeling of the immune microenvironment of HPV-negative head and neck cancer models.
    Article Snippet: Amplification and inhibition of microRNA Cells were cultured in complete culture media supplemented with recombinant IL-8 (1 ng/mL, R&D Systems, Minneapolis, MN) and either DMSO or SX-682 (5 μM) for 24 h (UM-SCC-11B and UM-SCC-22B) or 48 h (UMSCC-74B). .. Micro-RNA was purified using the PureLink miRNA Isolation Kit (Invitrogen), according to the manufacturer’s instructions; next, reverse transcription was performed with a TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems) in conjunction with miR-specific primers for hsa-mir-200a (ID: 000502), hsamir-200b (ID: 002251), hsa-mir-200c (ID: 002300), and snoRNA-135 (ID: 001230) (TaqMan MicroRNA Assay, Applied Biosystems). cDNA was amplified in triplicate using TaqMan Master Mix with no AMP UNG (ThermoFisher Scientific) in an Applied Biosystems 7500 RealTime PCR System (ThermoFisher Scientific). miRNA expression was normalized to snoRNA-135 by calculating 2−ΔCt. ..

    Polymerase Chain Reaction:

    Article Title: Inhibition of the chemokine receptors CXCR1 and CXCR2 synergizes with docetaxel for effective tumor control and remodeling of the immune microenvironment of HPV-negative head and neck cancer models.
    Article Snippet: Amplification and inhibition of microRNA Cells were cultured in complete culture media supplemented with recombinant IL-8 (1 ng/mL, R&D Systems, Minneapolis, MN) and either DMSO or SX-682 (5 μM) for 24 h (UM-SCC-11B and UM-SCC-22B) or 48 h (UMSCC-74B). .. Micro-RNA was purified using the PureLink miRNA Isolation Kit (Invitrogen), according to the manufacturer’s instructions; next, reverse transcription was performed with a TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems) in conjunction with miR-specific primers for hsa-mir-200a (ID: 000502), hsamir-200b (ID: 002251), hsa-mir-200c (ID: 002300), and snoRNA-135 (ID: 001230) (TaqMan MicroRNA Assay, Applied Biosystems). cDNA was amplified in triplicate using TaqMan Master Mix with no AMP UNG (ThermoFisher Scientific) in an Applied Biosystems 7500 RealTime PCR System (ThermoFisher Scientific). miRNA expression was normalized to snoRNA-135 by calculating 2−ΔCt. ..

    Expressing:

    Article Title: Inhibition of the chemokine receptors CXCR1 and CXCR2 synergizes with docetaxel for effective tumor control and remodeling of the immune microenvironment of HPV-negative head and neck cancer models.
    Article Snippet: Amplification and inhibition of microRNA Cells were cultured in complete culture media supplemented with recombinant IL-8 (1 ng/mL, R&D Systems, Minneapolis, MN) and either DMSO or SX-682 (5 μM) for 24 h (UM-SCC-11B and UM-SCC-22B) or 48 h (UMSCC-74B). .. Micro-RNA was purified using the PureLink miRNA Isolation Kit (Invitrogen), according to the manufacturer’s instructions; next, reverse transcription was performed with a TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems) in conjunction with miR-specific primers for hsa-mir-200a (ID: 000502), hsamir-200b (ID: 002251), hsa-mir-200c (ID: 002300), and snoRNA-135 (ID: 001230) (TaqMan MicroRNA Assay, Applied Biosystems). cDNA was amplified in triplicate using TaqMan Master Mix with no AMP UNG (ThermoFisher Scientific) in an Applied Biosystems 7500 RealTime PCR System (ThermoFisher Scientific). miRNA expression was normalized to snoRNA-135 by calculating 2−ΔCt. ..



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    Thermo Fisher purelink mirna isolation kit
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    Thermo Fisher purelink/ mirvana mirna isolation kit
    General overview of <t>miRNA</t> detection workflow. The steps are as follows: A. Sample collection from the patient, B. sample treatment, C. small RNA isolation including miRNA, D. sample processing including methods of target amplification, library preparation, and surface hybridisation, and E. detection of signature miRNA.
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    General overview of miRNA detection workflow. The steps are as follows: A. Sample collection from the patient, B. sample treatment, C. small RNA isolation including miRNA, D. sample processing including methods of target amplification, library preparation, and surface hybridisation, and E. detection of signature miRNA.

    Journal: Expert Reviews in Molecular Medicine

    Article Title: Review of microRNA detection workflows from liquid biopsy for disease diagnostics

    doi: 10.1017/erm.2025.2

    Figure Lengend Snippet: General overview of miRNA detection workflow. The steps are as follows: A. Sample collection from the patient, B. sample treatment, C. small RNA isolation including miRNA, D. sample processing including methods of target amplification, library preparation, and surface hybridisation, and E. detection of signature miRNA.

    Article Snippet: Thermal cycling amplification – Poly(A)-tailing/ Stem-loop RT-qPCR , Fluorescence – Probe based (Universal or miRNA specific) , Bacteria, Virus, Cells, Plant, Tissue, Yeast , Organic phase separation , PureLink/ mirVana miRNA Isolation Kit (Invitrogen) , TaqMan (Advanced) MicroRNA Assays (Applied Biosystems) , Custom , Custom , Target dependent , ThermoFisher Scientific.

    Techniques: Isolation, Amplification, Hybridization

    Comparison of clinically validated miRNA detection workflows. Sample type, treatment, RNA isolation, processing, and detection methods are outlined.

    Journal: Expert Reviews in Molecular Medicine

    Article Title: Review of microRNA detection workflows from liquid biopsy for disease diagnostics

    doi: 10.1017/erm.2025.2

    Figure Lengend Snippet: Comparison of clinically validated miRNA detection workflows. Sample type, treatment, RNA isolation, processing, and detection methods are outlined.

    Article Snippet: Thermal cycling amplification – Poly(A)-tailing/ Stem-loop RT-qPCR , Fluorescence – Probe based (Universal or miRNA specific) , Bacteria, Virus, Cells, Plant, Tissue, Yeast , Organic phase separation , PureLink/ mirVana miRNA Isolation Kit (Invitrogen) , TaqMan (Advanced) MicroRNA Assays (Applied Biosystems) , Custom , Custom , Target dependent , ThermoFisher Scientific.

    Techniques: Comparison, Isolation

    Overview of unique  miRNA  detection workflow for targeted disease diagnostics from liquid biopsies

    Journal: Expert Reviews in Molecular Medicine

    Article Title: Review of microRNA detection workflows from liquid biopsy for disease diagnostics

    doi: 10.1017/erm.2025.2

    Figure Lengend Snippet: Overview of unique miRNA detection workflow for targeted disease diagnostics from liquid biopsies

    Article Snippet: Thermal cycling amplification – Poly(A)-tailing/ Stem-loop RT-qPCR , Fluorescence – Probe based (Universal or miRNA specific) , Bacteria, Virus, Cells, Plant, Tissue, Yeast , Organic phase separation , PureLink/ mirVana miRNA Isolation Kit (Invitrogen) , TaqMan (Advanced) MicroRNA Assays (Applied Biosystems) , Custom , Custom , Target dependent , ThermoFisher Scientific.

    Techniques: Isolation, Amplification, Fluorescence, SYBR Green Assay, Clinical Proteomics, Purification, Bacteria, Virus, Extraction, Infection, Nucleic Acid Electrophoresis, Electrophoresis, cDNA Synthesis, Imaging, CRISPR, Centrifugation, Electrochemiluminescence, RNA Sequencing, Next-Generation Sequencing, Flow Cytometry, Microarray, Magnetic Beads

    Overview of the three common types of RNA isolation for miRNA detection. A. Organic phase separation-based extraction method where vesicles are lysed, and contaminants are separated by centrifugal force. B. Spin column-based extraction method with a similar lysis procedure but where RNA is bound and eluted through a silica filter and C. Magnetic bead-based extraction method where RNA is bound to silica-based magnetic nanoparticles through ionic bridges supported by cations. The RNA is eluted sequentially in a water-based solution. ( Washing steps not shown ).

    Journal: Expert Reviews in Molecular Medicine

    Article Title: Review of microRNA detection workflows from liquid biopsy for disease diagnostics

    doi: 10.1017/erm.2025.2

    Figure Lengend Snippet: Overview of the three common types of RNA isolation for miRNA detection. A. Organic phase separation-based extraction method where vesicles are lysed, and contaminants are separated by centrifugal force. B. Spin column-based extraction method with a similar lysis procedure but where RNA is bound and eluted through a silica filter and C. Magnetic bead-based extraction method where RNA is bound to silica-based magnetic nanoparticles through ionic bridges supported by cations. The RNA is eluted sequentially in a water-based solution. ( Washing steps not shown ).

    Article Snippet: Thermal cycling amplification – Poly(A)-tailing/ Stem-loop RT-qPCR , Fluorescence – Probe based (Universal or miRNA specific) , Bacteria, Virus, Cells, Plant, Tissue, Yeast , Organic phase separation , PureLink/ mirVana miRNA Isolation Kit (Invitrogen) , TaqMan (Advanced) MicroRNA Assays (Applied Biosystems) , Custom , Custom , Target dependent , ThermoFisher Scientific.

    Techniques: Isolation, Extraction, Lysis

    Schematic illustration of thermal amplification methods for miRNA detection. A. Stem-Loop RT-qPCR (Refs , , , , ) with (i) SYBR Green detection - universal and (ii) Probe-based detection - specific; B. Poly-A Tail RT-qPCR; (Refs , ) C. Sensitive and Multiplexed One-Step RT-qPCR (SMOS-qPCR); (Ref. ) and D. Droplet digital PCR (ddPCR). (Refs , )

    Journal: Expert Reviews in Molecular Medicine

    Article Title: Review of microRNA detection workflows from liquid biopsy for disease diagnostics

    doi: 10.1017/erm.2025.2

    Figure Lengend Snippet: Schematic illustration of thermal amplification methods for miRNA detection. A. Stem-Loop RT-qPCR (Refs , , , , ) with (i) SYBR Green detection - universal and (ii) Probe-based detection - specific; B. Poly-A Tail RT-qPCR; (Refs , ) C. Sensitive and Multiplexed One-Step RT-qPCR (SMOS-qPCR); (Ref. ) and D. Droplet digital PCR (ddPCR). (Refs , )

    Article Snippet: Thermal cycling amplification – Poly(A)-tailing/ Stem-loop RT-qPCR , Fluorescence – Probe based (Universal or miRNA specific) , Bacteria, Virus, Cells, Plant, Tissue, Yeast , Organic phase separation , PureLink/ mirVana miRNA Isolation Kit (Invitrogen) , TaqMan (Advanced) MicroRNA Assays (Applied Biosystems) , Custom , Custom , Target dependent , ThermoFisher Scientific.

    Techniques: Amplification, Quantitative RT-PCR, SYBR Green Assay, Digital PCR

    Schematic illustration of isothermal amplification methods for detecting miRNA. A. Rolling circle amplification (RCA) + APE1 enzyme; (Ref. ) B. Padlock-assisted hyperbranched rolling circle amplification (HRCA); (Ref. ) C. Primer-based loop-mediated isothermal amplification (PS-LAMP); (Ref. ) D. Catalytic assembly of DNAzyme integrated with primer exchange reaction (CDiPER); (Ref. ) and E. Multiplexed exponential amplification reaction (MEXPAR). (Ref. )

    Journal: Expert Reviews in Molecular Medicine

    Article Title: Review of microRNA detection workflows from liquid biopsy for disease diagnostics

    doi: 10.1017/erm.2025.2

    Figure Lengend Snippet: Schematic illustration of isothermal amplification methods for detecting miRNA. A. Rolling circle amplification (RCA) + APE1 enzyme; (Ref. ) B. Padlock-assisted hyperbranched rolling circle amplification (HRCA); (Ref. ) C. Primer-based loop-mediated isothermal amplification (PS-LAMP); (Ref. ) D. Catalytic assembly of DNAzyme integrated with primer exchange reaction (CDiPER); (Ref. ) and E. Multiplexed exponential amplification reaction (MEXPAR). (Ref. )

    Article Snippet: Thermal cycling amplification – Poly(A)-tailing/ Stem-loop RT-qPCR , Fluorescence – Probe based (Universal or miRNA specific) , Bacteria, Virus, Cells, Plant, Tissue, Yeast , Organic phase separation , PureLink/ mirVana miRNA Isolation Kit (Invitrogen) , TaqMan (Advanced) MicroRNA Assays (Applied Biosystems) , Custom , Custom , Target dependent , ThermoFisher Scientific.

    Techniques: Amplification

    Schematic illustration of isothermal amplification methods with CRISPR/Cas systems for detecting miRNA. A. Rolling circle amplification (RCA)-CRISPR/Cas9; (Ref. ) B. exponential amplification reaction (EXPAR)-CRISPR/Cas12a; (Ref. ) C. Strand displacement amplification (SDA)-CRISPR/Cas14a; (Ref. ) and D. Target-triggered SDA (ttSDA)-CRISPR/Cas12a + Pt@Au nanozymes. (Ref. )

    Journal: Expert Reviews in Molecular Medicine

    Article Title: Review of microRNA detection workflows from liquid biopsy for disease diagnostics

    doi: 10.1017/erm.2025.2

    Figure Lengend Snippet: Schematic illustration of isothermal amplification methods with CRISPR/Cas systems for detecting miRNA. A. Rolling circle amplification (RCA)-CRISPR/Cas9; (Ref. ) B. exponential amplification reaction (EXPAR)-CRISPR/Cas12a; (Ref. ) C. Strand displacement amplification (SDA)-CRISPR/Cas14a; (Ref. ) and D. Target-triggered SDA (ttSDA)-CRISPR/Cas12a + Pt@Au nanozymes. (Ref. )

    Article Snippet: Thermal cycling amplification – Poly(A)-tailing/ Stem-loop RT-qPCR , Fluorescence – Probe based (Universal or miRNA specific) , Bacteria, Virus, Cells, Plant, Tissue, Yeast , Organic phase separation , PureLink/ mirVana miRNA Isolation Kit (Invitrogen) , TaqMan (Advanced) MicroRNA Assays (Applied Biosystems) , Custom , Custom , Target dependent , ThermoFisher Scientific.

    Techniques: Amplification, CRISPR

    Schematic illustration of isothermal amplification methods with nanomaterials for detecting miRNA. A. Hybridisation chain reaction (HCR) + Molybdenum disulfide (MoS 2 ) nanosheets; (Ref. ) B. HCR + magnetic covalent organic framework (COF) nanospheres; (Ref. ) C. Catalytic hairpin assembly reaction (CHA) + HCR + Au-Ag nanoparticles; (Ref. ) and D. CHA + enzyme-assisted signal amplification (EASA) + Au–Ag nanoshuttles. (Ref. )

    Journal: Expert Reviews in Molecular Medicine

    Article Title: Review of microRNA detection workflows from liquid biopsy for disease diagnostics

    doi: 10.1017/erm.2025.2

    Figure Lengend Snippet: Schematic illustration of isothermal amplification methods with nanomaterials for detecting miRNA. A. Hybridisation chain reaction (HCR) + Molybdenum disulfide (MoS 2 ) nanosheets; (Ref. ) B. HCR + magnetic covalent organic framework (COF) nanospheres; (Ref. ) C. Catalytic hairpin assembly reaction (CHA) + HCR + Au-Ag nanoparticles; (Ref. ) and D. CHA + enzyme-assisted signal amplification (EASA) + Au–Ag nanoshuttles. (Ref. )

    Article Snippet: Thermal cycling amplification – Poly(A)-tailing/ Stem-loop RT-qPCR , Fluorescence – Probe based (Universal or miRNA specific) , Bacteria, Virus, Cells, Plant, Tissue, Yeast , Organic phase separation , PureLink/ mirVana miRNA Isolation Kit (Invitrogen) , TaqMan (Advanced) MicroRNA Assays (Applied Biosystems) , Custom , Custom , Target dependent , ThermoFisher Scientific.

    Techniques: Amplification, Hybridization

    Schematic illustration of isothermal amplification methods with metal–organic frameworks (MOFs) for detecting miRNA. A. Rolling circle amplification (RCA) + MOF-525; (Ref. ) B. Primer exchange reaction (PER) + MOF@Pt@MOF nanozyme; (Ref. ) C. Rolling hoop orbital amplification (RHOA) + Iron-Zirconium (Fe-Zr) MOF/G-quadruplex (G4) nanozyme; (Ref. ) and D. λ-Exonuclease + ZIF-67 MOF/ Tungsten(VI) oxide (WO 3 ) nanoflakes. (Ref. )

    Journal: Expert Reviews in Molecular Medicine

    Article Title: Review of microRNA detection workflows from liquid biopsy for disease diagnostics

    doi: 10.1017/erm.2025.2

    Figure Lengend Snippet: Schematic illustration of isothermal amplification methods with metal–organic frameworks (MOFs) for detecting miRNA. A. Rolling circle amplification (RCA) + MOF-525; (Ref. ) B. Primer exchange reaction (PER) + MOF@Pt@MOF nanozyme; (Ref. ) C. Rolling hoop orbital amplification (RHOA) + Iron-Zirconium (Fe-Zr) MOF/G-quadruplex (G4) nanozyme; (Ref. ) and D. λ-Exonuclease + ZIF-67 MOF/ Tungsten(VI) oxide (WO 3 ) nanoflakes. (Ref. )

    Article Snippet: Thermal cycling amplification – Poly(A)-tailing/ Stem-loop RT-qPCR , Fluorescence – Probe based (Universal or miRNA specific) , Bacteria, Virus, Cells, Plant, Tissue, Yeast , Organic phase separation , PureLink/ mirVana miRNA Isolation Kit (Invitrogen) , TaqMan (Advanced) MicroRNA Assays (Applied Biosystems) , Custom , Custom , Target dependent , ThermoFisher Scientific.

    Techniques: Amplification

    Schematic illustration of electrochemical and electrical nanoparticle miRNA detection methods. A. Magnetic microbeads & diblock oligonucleotide-modified Au nanoparticles; (Ref. ) B. Peptide nucleic acid (PNA) functionalised nanochannel biosensor; (Ref. ) C. DNA tetrahedral nanostructure-based biosensor; (Ref. ) D. Carbon nanotube biosensor; (Ref. ) and E. Phosphorodiamidate morpholino oligomers (PMO) - graphene quantum dots (GQDs) - functionalised reduced graphene oxide (RGO) field effect transistor (FET) biosensor. (Ref. )

    Journal: Expert Reviews in Molecular Medicine

    Article Title: Review of microRNA detection workflows from liquid biopsy for disease diagnostics

    doi: 10.1017/erm.2025.2

    Figure Lengend Snippet: Schematic illustration of electrochemical and electrical nanoparticle miRNA detection methods. A. Magnetic microbeads & diblock oligonucleotide-modified Au nanoparticles; (Ref. ) B. Peptide nucleic acid (PNA) functionalised nanochannel biosensor; (Ref. ) C. DNA tetrahedral nanostructure-based biosensor; (Ref. ) D. Carbon nanotube biosensor; (Ref. ) and E. Phosphorodiamidate morpholino oligomers (PMO) - graphene quantum dots (GQDs) - functionalised reduced graphene oxide (RGO) field effect transistor (FET) biosensor. (Ref. )

    Article Snippet: Thermal cycling amplification – Poly(A)-tailing/ Stem-loop RT-qPCR , Fluorescence – Probe based (Universal or miRNA specific) , Bacteria, Virus, Cells, Plant, Tissue, Yeast , Organic phase separation , PureLink/ mirVana miRNA Isolation Kit (Invitrogen) , TaqMan (Advanced) MicroRNA Assays (Applied Biosystems) , Custom , Custom , Target dependent , ThermoFisher Scientific.

    Techniques: Modification

    Schematic illustration of fluorescent, plasmonic, and colourimetric nanoparticle miRNA detection methods. A. Lanthanum oxide (La(III))-metal–organic framework (MOF) & silver (Ag) nanoparticle biosensor; (Ref. ) B. Iron(II,III) oxide@Titanium oxide (Fe 3 O 4 @TiO 2) nanoparticle biosensor; (Ref. ) C. Gold (Au) nanoprism biosensor;(Ref. ) and D. Gold (Au) nanoparticle liposome-based microfluidic chip biosensor. (Ref. )

    Journal: Expert Reviews in Molecular Medicine

    Article Title: Review of microRNA detection workflows from liquid biopsy for disease diagnostics

    doi: 10.1017/erm.2025.2

    Figure Lengend Snippet: Schematic illustration of fluorescent, plasmonic, and colourimetric nanoparticle miRNA detection methods. A. Lanthanum oxide (La(III))-metal–organic framework (MOF) & silver (Ag) nanoparticle biosensor; (Ref. ) B. Iron(II,III) oxide@Titanium oxide (Fe 3 O 4 @TiO 2) nanoparticle biosensor; (Ref. ) C. Gold (Au) nanoprism biosensor;(Ref. ) and D. Gold (Au) nanoparticle liposome-based microfluidic chip biosensor. (Ref. )

    Article Snippet: Thermal cycling amplification – Poly(A)-tailing/ Stem-loop RT-qPCR , Fluorescence – Probe based (Universal or miRNA specific) , Bacteria, Virus, Cells, Plant, Tissue, Yeast , Organic phase separation , PureLink/ mirVana miRNA Isolation Kit (Invitrogen) , TaqMan (Advanced) MicroRNA Assays (Applied Biosystems) , Custom , Custom , Target dependent , ThermoFisher Scientific.

    Techniques:

    Schematic illustration of various miRNA detection methods. A. Microarray, (Ref. ) B. nCounter, (Refs , ) C. RNA sequencing, (Ref. ) and D. Capillary electrophoresis. (Ref. )

    Journal: Expert Reviews in Molecular Medicine

    Article Title: Review of microRNA detection workflows from liquid biopsy for disease diagnostics

    doi: 10.1017/erm.2025.2

    Figure Lengend Snippet: Schematic illustration of various miRNA detection methods. A. Microarray, (Ref. ) B. nCounter, (Refs , ) C. RNA sequencing, (Ref. ) and D. Capillary electrophoresis. (Ref. )

    Article Snippet: Thermal cycling amplification – Poly(A)-tailing/ Stem-loop RT-qPCR , Fluorescence – Probe based (Universal or miRNA specific) , Bacteria, Virus, Cells, Plant, Tissue, Yeast , Organic phase separation , PureLink/ mirVana miRNA Isolation Kit (Invitrogen) , TaqMan (Advanced) MicroRNA Assays (Applied Biosystems) , Custom , Custom , Target dependent , ThermoFisher Scientific.

    Techniques: Microarray, RNA Sequencing, Electrophoresis